Course Information
Course Semester Plan
Course Information
| No | Module name | DNA and Protein Recombinant Technology |
| 1 | Courses code | P20.02021 |
| 2 | Study loads | 3 credits |
| 3 | Semester | Second Semester |
| 4 | Precondition | there is no |
| 5 | Competence | Upon completion of this course, students are expected to be able to: 1. describe the physical and chemical properties of compounds that affect solubility, stability, and other biopharmaceutical properties / behaviors used in the development of drug dosage form from natural ingredients 2. describes and applies the mechanism of a particular drug at the molecular and cellular levels 3. Apply different instrumental analytical techniques to the analysis of natural drugs for the evaluation of their pharmacological effects 4. Shows individual contributions to a project or group assignment 5. Summarize information gained from the group experience and communicate its development |
| 6 | Elements of competency | MKK |
| 7 | Type competency | Supporting competence |
| 8 | Syllabus | 1. Prokaryotes and eukaryotes genetic information flow; 2. plasmids and vectors including the type of plasmids/ vector, genetics and the function of the plasmids/ vectors; 3. Regulation of recombinant gene expression in prokaryotes: components of transcription and translation and mechanisms of gene regulation; 4. Target DNA preparation: genome isolation, primer design, and DNA amplification by PCR; 5. Preparation of target DNA with synthetic gene design; 6. Gene cloning includes: target DNA ligation with vectors, transformation of recombinant plasmids and characterization of recombinant clones; 7. DNA target construction -vector expression, gene over-expression and protein overproduction; 8. Mid-term Exam; 9. Recombinant protein purification and characterization; 10. Amino acids and peptides: fundamental components of protein, the chemical properties of polypeptides and the acids-bases properties of amino acids; 11. Protein: protein structure, protein folding and protein structural motif; 12. Prediction of protein structure: homologous protein, prediction of tertiary structure and threading method; 13. Protein engineering: engineering for protein stabilization, enzymatic activity and specificity; 14. Mutagenesis: site-directed and random mutagenesis methods; 15. Mutant protein: design mutant protein with site-directed mutagenesis; 16. Final Exam |
| 9 | Attribute to soft skills | ethics, discipiline, awareness, communication skill |
| 10 | Learning methods | Lecture and Discussion |
| 11 | Learning media | LCD/projector, White board and Hand out |
| 12 | Appraisal | Written Test and Presentation |
| 13 | Lecturer | Dr. Apt.Tina Rostinawati, M.Si. Dr. Apt. Tiana Milanda, M.Si., and Apt. Arif Satria, M.Si. |
| 14 | References | Crommelin DJA, Sindelar RD, Meibohm B. Pharmaceutical biotechnology: Fundamentals and application. 5th ed. Springer. 2019. Bhatia S, Goli D. Introduction to Pharmaceutical Biotechnology: basic tachniques and concepts. Vol 1. IOP publishing Ltd. 2018. Clark DP, Pazdernik NJ. Biotechnology. Amsterdam: Elsevier/Academic Cell Press, 2016. |
Course Semester Plan