Screening for PPAR γ agonist from Myristica fragrans Houtt seeds for the treatment of Type 2 diabetes by in vitro and in vivoScreening for PPAR γ agonist from Myristica fragrans Houtt seeds for the treatment of Type 2 diabetes by in vitro and in vivo

Keri Lestari*, JK Hwang, Sri Hartini Kariadi, Andi Wijaya, Trihanggono Ahmad, Anas Subarnas, Supriyatna, M. Muchtaridi

Med. Health Sc. J. 2012: 12 (3) 

Correspondence : lestarikd@unpad.ac.id

In general, a type two diabetes mellitus (T2DM) patient suffers for hyperglycaemia and insulin resistance. Nowadays, there is an anti diabetes drug working as a PPAR gamma/alpha. agonist and has a significant effect to improve the hyperglycemia and insulin resistance condition. However, this drug gives a negative effect on lipid circulation parameters. In this investigation, nutmeg (Myristica fragrans) extracts (NuSE) was tested against PPARγ using cell-based GAL4/PPAR chimera transactivation and trancient transvection assay with three PPAR response element (PPREs) containing reporters. The results demonstrate that the NuSE has significant PPARγ agonist activity, although their potency was less than the standard PPARγ full agonist. An investigation of antidiabetic activity of the NuSE has been carried out on rats. Rats were treated orally every day for six days with the ethanol of NuSE in several doses. The result of the experiment showed that each dose of NuSE gave hypoglycemic activity (p= 0.01 and p=0.05). The experiment also showed that an increase in dosage caused an increased in the hypoglycemic activity. Therefore, nutmeg (Myristica fragrans) seeds might be potential against antidiabetic agent for the treatment of type two diabetes with a PPAR  gamma/alpha. agonistic mechanism.

avaliable at http://academicpublishingplatforms.com/article.php?journal=MHSJ&number=12&article=1621Keri Lestari*, JK Hwang, Sri Hartini Kariadi, Andi Wijaya, Trihanggono Ahmad, Anas Subarnas, Supriyatna, M. Muchtaridi

Med. Health Sc. J. 2012: 12 (3) 

Correspondence : lestarikd@unpad.ac.id

In general, a type two diabetes mellitus (T2DM) patient suffers for hyperglycaemia and insulin resistance. Nowadays, there is an anti diabetes drug working as a PPAR gamma/alpha. agonist and has a significant effect to improve the hyperglycemia and insulin resistance condition. However, this drug gives a negative effect on lipid circulation parameters. In this investigation, nutmeg (Myristica fragrans) extracts (NuSE) was tested against PPARγ using cell-based GAL4/PPAR chimera transactivation and trancient transvection assay with three PPAR response element (PPREs) containing reporters. The results demonstrate that the NuSE has significant PPARγ agonist activity, although their potency was less than the standard PPARγ full agonist. An investigation of antidiabetic activity of the NuSE has been carried out on rats. Rats were treated orally every day for six days with the ethanol of NuSE in several doses. The result of the experiment showed that each dose of NuSE gave hypoglycemic activity (p= 0.01 and p=0.05). The experiment also showed that an increase in dosage caused an increased in the hypoglycemic activity. Therefore, nutmeg (Myristica fragrans) seeds might be potential against antidiabetic agent for the treatment of type two diabetes with a PPAR  gamma/alpha. agonistic mechanism.

avaliable at http://academicpublishingplatforms.com/article.php?journal=MHSJ&number=12&article=1621

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