ANTI-MALARIAL COMPOUND FROM THE STEM BARK OF Erythrina variegataANTI-MALARIAL COMPOUND FROM THE STEM BARK OF Erythrina variegata

Tati Herlina1*, Unang Supratman1, Ukun M.S. Soedjanaatmadja1, Anas Subarnas2, Supriyatna
Sutardjo2, Noor Rain Abdullah3, and Hideo Hayashi4

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Jatinangor 45363, Sumedang,
Indonesia
2Faculty of Pharmacy, Padjadjaran University, Jatinangor 45363, Sumedang, Indonesia
3Herbal Medicine Research Center, Institute for Medical Research, Jalan Pahang 50588,Kuala Lumpur, Malaysia
4Laboratory of Natural Products Chemistry, Division of Applied Biological Chemistry, Graduated School of Agriculture and Life
Sciences,Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan
Received May 5, 2009; Accepted July 16, 2009

ABSTRACT
During the course of our continuing search for novel anti-malarial compounds from Indonesian plants, the
methanol extract of the bark of E. variegata showed significant anti-malarial activity toward Plasmodium falciparum
in vitro using the lactate dehydrogenase (LDH) assay. The methanol extract of the bark of E. variegata was
separated by using bioassay-guide fractionation. The ethyl acetate fraction showed the most activity, exhibiting
equipotency against both strains of parasite with IC50 of 23.8 μg/mL against 3D7 and 9.3 μg/mL against K1.
Furthermore, by using the anti-malarial activity to follow separation, the ethyl acetate fraction was separated by
combination of column chromatography to yield an active compound. The chemical structure of active compound
was determined on the basis of spectroscopic evidences and comparison with those previously reported and
identified as an isoflavonoid, warangalone. The warangalone showed anti-malarial activity against both strains of
parasite used with IC50 of 4.8 μg/mL against 3D7 and 3.7 μg/mL against K1.
Keywords: Antimalarial, Erythrina variegata, warangalone

Availabel at http://pdm-mipa.ugm.ac.id/ojs/index.php/ijc/article/viewArticle/526Tati Herlina1*, Unang Supratman1, Ukun M.S. Soedjanaatmadja1, Anas Subarnas2, Supriyatna
Sutardjo2, Noor Rain Abdullah3, and Hideo Hayashi4

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Jatinangor 45363, Sumedang,
Indonesia
2Faculty of Pharmacy, Padjadjaran University, Jatinangor 45363, Sumedang, Indonesia
3Herbal Medicine Research Center, Institute for Medical Research, Jalan Pahang 50588,Kuala Lumpur, Malaysia
4Laboratory of Natural Products Chemistry, Division of Applied Biological Chemistry, Graduated School of Agriculture and Life
Sciences,Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan
Received May 5, 2009; Accepted July 16, 2009

ABSTRACT
During the course of our continuing search for novel anti-malarial compounds from Indonesian plants, the
methanol extract of the bark of E. variegata showed significant anti-malarial activity toward Plasmodium falciparum
in vitro using the lactate dehydrogenase (LDH) assay. The methanol extract of the bark of E. variegata was
separated by using bioassay-guide fractionation. The ethyl acetate fraction showed the most activity, exhibiting
equipotency against both strains of parasite with IC50 of 23.8 μg/mL against 3D7 and 9.3 μg/mL against K1.
Furthermore, by using the anti-malarial activity to follow separation, the ethyl acetate fraction was separated by
combination of column chromatography to yield an active compound. The chemical structure of active compound
was determined on the basis of spectroscopic evidences and comparison with those previously reported and
identified as an isoflavonoid, warangalone. The warangalone showed anti-malarial activity against both strains of
parasite used with IC50 of 4.8 μg/mL against 3D7 and 3.7 μg/mL against K1.
Keywords: Antimalarial, Erythrina variegata, warangalone

Availabel at http://pdm-mipa.ugm.ac.id/ojs/index.php/ijc/article/viewArticle/526

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